Journal:

Article Title: Normal T-Cell Turnover in Sooty Mangabeys Harboring Active Simian Immunodeficiency Virus Infection

doi:

Figure Lengend Snippet: Comparison of T-lymphocyte subsets and proliferation rates in sooty mangabeys and rhesus macaques

Article Snippet: For intracellular staining, 100 μl of whole blood was incubated with the antibodies to surface antigens CD3-PE (clone SP34; Pharmingen), CD4-APC, and CD8-PerCP for 15 min, after which they were fixed and permeabilized for 40 min at RT with 2 ml of the Permeafix reagent (Ortho Diagnostic Systems).

Techniques:

Journal:

Article Title: Normal T-Cell Turnover in Sooty Mangabeys Harboring Active Simian Immunodeficiency Virus Infection

doi:

Figure Lengend Snippet: Preferential expression of Ki-67 in the CD45RA− subset of T lymphocytes. (A) Combined analysis of Ki-67 and CD45RA expression by flow cytometry. Dot plots showing the staining for Ki-67 (x axis) and CD45RA (y axis) in the CD3+ CD8+ T-cell population of two representative animals. Ki-67 is expressed preferentially in the CD45RA− memory T-cell subset, both in an infected mangabey (left panel) and in an infected macaque (right panel). The numbers in the corners of the plots indicate the percentage of CD3+ CD8+ T cells in each quadrant. (B) Percentage of Ki-67+ cells within CD45RA+ and CD45RA− T-cell subsets. Graphs show results for four types of cells, as follows: B-1, mangabey CD4+ T cells; B-2, macaque CD4+ T cells; B-3, mangabey CD8+ T cells; B-4, macaque CD8+ T cells. Only the P values lower than 0.05 in the nonparametric Mann-Whitney test are reported. SIV−, SIV-negative; SIV+, SIV-positive.

Article Snippet: For intracellular staining, 100 μl of whole blood was incubated with the antibodies to surface antigens CD3-PE (clone SP34; Pharmingen), CD4-APC, and CD8-PerCP for 15 min, after which they were fixed and permeabilized for 40 min at RT with 2 ml of the Permeafix reagent (Ortho Diagnostic Systems).

Techniques: Expressing, Flow Cytometry, Staining, Infection, MANN-WHITNEY

Journal:

Article Title: Normal T-Cell Turnover in Sooty Mangabeys Harboring Active Simian Immunodeficiency Virus Infection

doi:

Figure Lengend Snippet: α1 circle numbers expressed in macaques. The number of α1 circles per million CD3+ T cells is reported as a function of the age of the animals (in years). Solid squares, SIV-positive macaques; open squares, uninfected macaques. The dashed line and the solid line correspond to regression lines obtained for uninfected and infected macaques, respectively. The slope of the regression line was s = −0.073 for uninfected macaques (P = 0.02). The slope did not significantly differ from 0 for SIV-positive macaques.

Article Snippet: For intracellular staining, 100 μl of whole blood was incubated with the antibodies to surface antigens CD3-PE (clone SP34; Pharmingen), CD4-APC, and CD8-PerCP for 15 min, after which they were fixed and permeabilized for 40 min at RT with 2 ml of the Permeafix reagent (Ortho Diagnostic Systems).

Techniques: Infection